I had a similar issue with my FT-2900R. I was able to talk fine the first night and then I went and programmed a bunch of channels and nobody was replying to me. I had the freq, offset and PL/CTSS code set correctly, but I did not have the Squelch Type turned on. I changed that from OFF to TONE and I could transmit again.
Good luck and I hope to hear you out there!
84 FJ60 The TuRDle
& '89 Pickup Ext Cab DLX 22RE/5spd