Yes, you can assume his old call sign assuming he has passed away or stopped renewing it and no one else has claimed it. Check the FCC website under the vanity call sign program. You as a relative do have a special access to the call sign (if for example you present a death certificate) but after a period of time it would become available to anyone.
'91 Pickup - Imelda
'08 Tacoma TRD - Donna
'09 Kawasaki KLR650
'12 Gunnar Rockhound 29
"To live is to suffer, to survive is to find some meaning in the suffering." -- Friedrich Nietzsche